Magenetic Resin - Dotway Biotech -- Mfg for recombinant protein and chromatography resin

Ni-NTA Magnetic Resin

Compared to its corresponding column chromatography agarose resin (Ni-NTA NUPharose FF, this product features the same ligand modification process and maintains a good balance between affinity (capacity) and specificity (selectivity): while the binding capacity for most His-tagged proteins exceeds 40 mg/mL, sample purity can reach over 90% after a single purification step, with low Ni2+ leakage in the eluate (Ni2+ to target protein molar ratio <0.1). The tetra-coordinate structure formed by the NTA ligand and Ni2+ in this product is stable. After chelation, it can tolerate certain concentrations of chelating agents, reducing agents, and alkali. When samples contain 1 mM EDTA, 1~10 mM β-mercaptoethanol, or 5 mM DTT, purification can proceed normally with Ni-NTA NUPharose Mag, followed by cleaning with 0.1~1 M NaOH.

Streptavidin Magnetic Resin

Streptavidin NUPharose Mag is an affinity magnetic resin. Its ligand is Streptavidin, and the NUPharose Mag base resins are agarose gel microspheres containing paramagnetic material. This magnetic resin product utilizes the specific binding between streptavidin and biotin to isolate and purify biotinylated antigens, antibodies, nucleic acids, and other substances from biological samples or mixtures. 1. Direct purification or enrichment of biotinylated biomolecules. 2. Purification of antigens or antibodies (Immuno recipitation). 3. Purification of 2-iminobiotin-labeled biomolecules.

Ni-TED Magnetic Resin

Ni-TED NUPharose Mag is a metal chelate affinity magnetic bead product primarily used for the rapid screening and purification of proteins with a histidine tag (His-tag). Its ligand is tris(carboxymethyl)ethylenediamine (TED) groups, the chelated metal ion is Ni2+, and the NUPharose Mag base matrix consists of agarose gel microspheres containing paramagnetic material. Compared to its corresponding column chromatography agarose resin (Ni-TED NUPharose FF), this product features the same ligand and maintains a good balance between affinity (capacity) and specificity (selectivity): while the binding capacity for His-tagged proteins exceeds 20 mg/mL, sample purity can reach over 90% after a single purification step, with Ni2+ content in the eluate comparable to environmental background levels, indicating extremely low metal ion leakage.

NHS Pre-activated Magnetic Resin

NHS Activated NUPharose Mag is a pre-activated magnetic resin product. The surface of the paramagnetic agarose gel microsphere matrix is modified with a ten-atom-long spacer arm, followed by activation of the carboxyl groups with N-hydroxysuccinimide (NHS). The NHS-activated groups can couple with functional biomacromolecules containing primary amines, such as antibodies, proteins, enzymes, and peptides, to form stable amide bonds. The resulting affinity magnetic beads can specifically bind target molecules, allowing high-purity product isolation in a single purification step. Compared to column chromatography with agarose resins, with below advantages: * Clarification of the feedstock is generally not required, shortening purification time and offering better enrichment effects for low-expression feedstocks; * Both manual and automated batch operations are possible. Manual operation is equipment-independent and offers high operational throughput.

r-Protein G Magnetic Resin

ProG NUPharose Mag is an antibody affinity magnetic resin product for the rapid and convenient separation and purification of various mammalian antibody subtypes or genetically engineered recombinant proteins containing antibody Fc fragments from ascites, serum, cell culture supernatants, or cell extracts. The ligand of this product is self-developed recombinant streptococcal Protein G , expressed in E. coli and free of animal-derived components. Compared to the Protein A ligand, the advantage of the Protein G ligand is its ability to bind to all human and mouse IgG subtypes. The NUPharose Mag base matrix consists of agarose gel microspheres containing paramagnetic material. The ligand is coupled via a single-point attachment, enhancing its binding capability to antibodies. Compared to its corresponding agarose resin (ProG NUPharose FF), this product features the same ligand and offers similar or higher binding capacity, with a binding capacity for h-IgG exceeding 40 mg/mL, characterized by high binding capacity.

Alkaline-Tolerant Protein A (rat-PA) Magnetic Resin

rat-PA NUPharose Mag is an antibody affinity magnetic resin product designed for the rapid and convenient separation and purification of various mammalian antibody subtypes or genetically engineered recombinant proteins containing the antibody Fc fragment from ascites, serum, cell culture supernatant, or cell extracts. Compared to column chromatography with below advantages: * Sample liquids generally do not require clarification, shortening purification time and providing better enrichment effects for low-expression samples; *Manual or automated batch operations are possible; manual operation is equipment-independent and offers high throughput. Therefore, this product is suitable for small-scale antibody purification and enrichment (e.g., hundred-milligram level). High-throughput experiments can quickly screen purification conditions and optimize results, and the product is reusable and applicable for large-scale bioseparation and purification. Additionally, magnetic bead products are also widely used in scenarios such as immunoprecipitation and pull-down assays.

r-Protein A Magnetic Resin

Compared to the corresponding agarose media (ProA NUPharose FF), this product has the same ligand, similar or higher binding capacity, with a binding capacity for human IgG exceeding 45 mg/mL. Compared to column chromatography operations with agarose media, in addition to retaining the characteristics of high capacity, high purity, high yield, and high reproducibility of antibody affinity chromatography, purification operations using magnetic media offer the following features and advantages: Feedstock generally does not require clarification, which can shorten purification time and provides better enrichment for low-expression feedstocks; Feedstock generally does not require clarification, which can shorten purification time and provides better enrichment for low-expression feedstocks. Therefore, this product is suitable for small-scale antibody purification and enrichment (e.g., hundreds of milligrams). High-throughput experiments can rapidly screen purification conditions and optimize results, and the resin is reusable and applicable for large-scale biological separation and purification.

Phenyl Hydrophobic Resin

Phenyl NUPharose FF and Phenyl NUPharose FF (LS) are hydrophobic chromatography media formed by coupling phenyl groups to agarose gel microspheres via stable ether bonds. The phenyl group is strongly hydrophobic and exhibits hydrophobic and π-π interactions with aromatic amino acid residues, making it suitable for target protein capture and intermediate purification. Phenyl NUPharose FF has a higher ligand density than Phenyl NUPharose FF(LS), resulting in different hydrophobicity and selectivity towards samples. Users can choose the appropriate product model based on specific application scenarios. Phenyl NUPharose HP is a high-resolution phenyl resin (HP stands for High Performance). Phenyl NUPharose HP features high resolution and is typically used in the polishing stage. Typical applications of phenyl resins include the production of recombinant human granulocytemacrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (GCSF), human macrophage colony-stimulating factor (M-CSF), epidermal growth factor (EGF), and the purification of monoclonal antibodies.

CM Ion Exchange Resin

Carboxymethyl (CM) is a weak cation exchanger. Compared to strong cation exchange (SP) media, weak cation exchange media offer different selectivity and can be considered when SP media fail to provide the required selectivity. Based on resolution and application, the CM NUPharose series can be divided into two products: CM NUPharose FF (~90 μm) and CM NUPharose HP (~35 μm). FF stands for Fast Flow. CM NUPharose FF features fast flow rates, suitable for rapid protein purification, wide applicability, and ease of scale-up. HP stands for High Performance. CM NUPharose HP offers high resolution and is often used in polishing stages.

IDA & Ni-IDA Metal Chelate Affinity Resin

Ni-IDA NUPharose FF and IDA NUPharose FF are metal chelate affinity chromatography resin containing iminodiacetic acid (IDA) groups, suitable for the affinity chromatography of most histidine-tagged (His-tag) proteins. Both products are based on our classic agarose gel microspheres, and “FF” stands for Fast Flow, indicating the resin can operate efficiently at high flow rates. IDA NUPharose FF is supplied without prechelated metal ions, allowing users to chelate metal ions such as Ni2+, Co2+, Cu2+, Zn2+ before use. Ni-IDA NUPharose FF is pre-chelated with Ni2+ and can be used directly. Through a unique ligand modification process, these products achieve a balance between affinity and specificity: binding capacity for most His-tagged proteins is greater than 40 mg/mL while achieving over 90% purity in a single-step purification.

Butyl Hydrophobic Resin

Butyl NUPharose FF is a hydrophobic chromatography medium with moderately strong hydrophobicity, formed by coupling n-butyl groups to agarose gel microspheres via stable ether bonds. Butyl NUPharose HP is a high-resolution butyl resin (HP stands for High Performance), which features high resolution and is typically used in the polishing stage. The n-butyl group is a “pure hydrophobic” group and exhibits selectivity differences towards various hydrophobic amino acid residues compared to phenyl groups. This product retains the excellent hydrophilicity and large network structure of agarose, offering good compatibility with bioactive macromolecules, high capacity, low non-specific adsorption, and high flow rates. It is widely used for laboratory-scale preparation of biomacromolecules such as proteins and peptides with certain hydrophobic properties, as well as for industrial-scale production in biopharmaceuticals and bioengineering.

NuPore 30, NuPore 75 and NuPore 200 Size Exclusion Resin

The NuPore series products are high-performance size exclusion resins formed by coupling dextran to a cross-linked agarose base matrix. These composite resin microspheres have a mean particle size of approximately 35 μm, combining the excellent mechanical properties of agarose with the high selectivity of the dextran network. Compared to traditional size exclusion resins, the NuPore series offers advantages such as high rigidity, high resolution, and high flow rates. They can be used for group separations like desalting and buffer exchange, as well as for fractionation processes of biomacromolecules such as peptides, proteins, nucleic acids with similar molecular weights, and components of natural products.

Co-NTA Metal Chelate Affinity Resin

Co-NTA NUPharose FF is a metal chelate resin containing nitrilotriacetic acid (NTA) groups, primarily used for the affinity chromatography of His-tagged proteins. This product is based on our classic NUPharose agarose gel microspheres, pre-chelated with Co2+ from IMAC NUPharose FF , and is ready for direct use by the user. Different metal ions have varying affinity and specificity for target proteins: the affinity of Cu2+, Ni2+, Zn2+, and Co2+ decreases sequentially (affecting yield), while specificity increases sequentially (affecting purity). This product, chelated with Co2+, can be considered when Ni2 +-chelated resins fail to meet purity requirements. Compared to iminodiacetic acid (IDA) ligands, NTA ligands offer more balanced performance, better tolerance to chelating agents, reducing agents, alkali, etc., and exhibit lower metal ion leaching during the purification process.

4FF and 6FF Gel Filtration Resin

NUPharose 4FF and NUPharose 6FF are agarose-based gel filtration resins from our classic NUPharose technology platform. Gel filtration resins are also referred to as size exclusion resins. NUPharose 4FF and NUPharose 6FF consist of 4% and 6% crosslinked agarose, respectively, with unique pore sizes and pore size distributions. Thanks to strict raw material screening and control, as well as innovations in bead forming and cross-linking processes, these products exhibit excellent pressure/flow characteristics and low non-specific adsorption to biomacromolecules, resulting in high product recovery. They are particularly suitable for the separation of larger particles such as viral particles and exosomes. Furthermore, NUPharose 4FF and NUPharose 6FF possess good physical and chemical stability. They can serve as base matrices and, after modification with specific small molecule or macromolecule ligands, can be used in various bioseparation processes.

NUPhadex G-10M, 15M & 50M Size Exclusion Resin

The NUPhadex G-10 M, NUPhadex G-15 M, and NUPhadex G-50 M series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. The dry powder particle sizes of these three products are similar. After swelling, the microspheres are rigid, with decreasing crosslinking density, increasing swelling factor for the dry powder, and increasing globular protein exclusion limits in the order G-10 M < G-15 M < G-50 M. Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product series suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids.

DEAE Ion Exchange Resin

The DEAE NUPharose series are weak anion exchange resins, where DEAE stands for Diethylaminoethyl. Compared to strong anion exchange (Q) resins, weak anion exchange resins offer different selectivity and can be considered when Q resins do not meet selectivity requirements. Based on resolution and application scenarios, the DEAE NUPharose series can be divided into two products: DEAE NUPharose FF (~90 μm) and DEAE NUPharose HP (~35 μm). FF stands for Fast Flow; DEAE NUPharose FF features fast flow rates suitable for rapid protein purification, wide applicability, and easy scale-up. HP stands for High Performance; DEAE NUPharose HP offers high resolution and is often used in polishing stages.

NUPhadex G-25 Size Exclusion Resin

The NUPhadex G-25 series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. These products have an exclusion limit of approximately 5000 Da for globular proteins. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids. It can also be used for fractionation processes to separate and purify biomolecules with small molecular weight differences, such as peptides, oligonucleotides, antibiotics, and natural products.

NUPhadex G-75M, 100M, 150M and 200M Size Exclusion Resin

The NUPhadex series size exclusion resins are epichlorohydrin-crosslinked dextran microspheres. Dextran exhibits extremely low non-specific adsorption to biomolecules. These hydrophilic, porous crosslinked dextran microspheres are primarily used for rapid buffer exchange and desalting of biomolecules in group separation processes, featuring minimal sample dilution, high desalting efficiency, and high recovery. Relatively large biomolecules have short retention times in the resin, while salts and other small molecules in the buffer are retained longer. Molecules of different sizes are separated based on their differing residence times in the resin, making this product suitable for various biomolecular samples such as proteins, polysaccharides, and nucleic acids. The globular protein exclusion limits increase progressively for NUPhadex G-75 M, NUPhadex G-100 M, NUPhadex G-150 M, and NUPhadex G-200 M. These four products are not rigid microspheres; the column bed is compressible and they are typically used with gravity flow.

4B and 6B Gel Filtration Resin

NUPharose 4B and NUPharose 6B are agarose-based gel filtration resins from the NUPharose technology platform. Gel filtration resins are also referred to as size exclusion resins. NUPharose 4B and NUPharose 6B consist of 4% and 6% agarose, respectively. Different concentrations of agarose solutions form different pore sizes upon gelation. The refined agarose has charged impurities removed, resulting in very low non-specific adsorption. These products are suitable for separating biomolecules of various molecular weights. Additionally, NUPharose 4B and NUPharose 6B can be activated for coupling different affinity ligands. we also offer epoxy-activated NUPharose 6B (Epoxy Activated NUPharose 6B).

r-Protein A Affinity Resin

The rProtein A ligand is expressed in E. coli, ensuring that it is free from animal-derived components while retaining all five binding domains of the wild-type protein. ProA NUPharose FF exhibits significantly higher binding capacity compared to wild-type Protein A resins, with a dynamic binding capacity for human IgG exceeding 40 mg/mL. This resin demonstrates affinity towards various types and subtypes of antibodies from multiple species, including human, mouse, rat, rabbit, and bovine sources. It is capable of isolating and purifying different subclasses of mammalian antibodies or genetically engineered recombinant proteins containing Fc fragments from ascites, serum, cell culture supernatants, or cell lysates, thereby meeting research and production requirements at various scales.

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